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American Society of Microbiology ABMM Sample Questions:

1. A research laboratory is studying the pathogenesis of respiratory syncytial virus (RSV) infection in infants.
They find that a specific viral surface glycoprotein is primarily responsible for attachment to host cells. This glycoprotein is:

A) Matrix protein (M)
B) Neuraminidase (N)
C) Fusion protein (F)
D) Hemagglutinin (H)

2. A clinical microbiology laboratory is implementing whole-genome sequencing (WGS) for enhanced surveillance of Listeria monocytogenes isolates associated with foodborne outbreaks. WGS provides several advantages over traditional subtyping methods like pulsed-field gel electrophoresis (PFGE). Which of the following is the MOST significant advantage of WGS in this context?

A) Higher discriminatory power for resolving phylogenetic relationships and identifying outbreakclusters.
B) Lower cost per isolate compared to PFGE.
C) Easier standardization and inter-laboratory comparison of data.
D) Faster turnaround time for obtaining results.

3. A patient develops severe, watery diarrhea shortly after starting a course of clindamycin. Stool testing using a cytotoxicity assay is positive. This result is most consistent with infection by which organism?

A) Campylobacter jejuni
B) Giardia lamblia
C) Clostridioides difficile
D) Norovirus

4. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate recovered from a patient with meningitis. The isolate shows reduced susceptibility to penicillin (MIC = 2 µg/mL). The MOST likely mechanism for this reduced susceptibility is:

A) Production of beta-lactamase enzymes that hydrolyze penicillin.
B) Alterations in the penicillin-binding proteins (PBPs) that reduce their affinity for penicillin.
C) Modification of the peptidoglycan structure, preventing penicillin binding.
D) Increased activity of efflux pumps that actively remove penicillin from the cell.

5. A patient develops a severe diarrheal illness after consuming raw oysters. Stool cultures grow a Gram- negative, comma-shaped bacterium that is oxidase-positive and grows on thiosulfate-citrate-bile salts-sucrose (TCBS) agar, producing green colonies. The MOST likely virulence factor contributing to the watery diarrhea is:

A) Heat-labile toxin (LT)
B) Shiga toxin
C) Cytotoxin-associated gene A (CagA)
D) Cholera toxin-like enterotoxin

Solutions: